The textbook gives the concept and reveals the main components of the content of the legal regime for handling material evidence in pre-trial proceedings. The species diversity of forms of implementation of the legal regime is presented, and methodological recommendations addressed to investigators on establishing the form of implementation of legal regimes depending on the species diversity of physical evidence are presented. The legislation is listed as of July 1, 2020. The manual is intended for employees of investigative bodies of the Investigative Committee of the Russian Federation, can be used in the educational process on the subject "criminal procedure"and when conducting classes in the framework of professional training.